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KMID : 0377619970620110909
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Korean Jungang Medical Journal
1997 Volume.62 No. 11 p.909 ~ p.909
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Experimentally-induced intrauterine infection causes fetal brain white matter lesions in rabbits
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Park, Kyo Hoon
Choi, Jin/Yoon, Bo Hyun/Jun, Jong Kwan/Choi, Seok Tae/Syn, Hee Chul/Chi, Je Geun
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Abstract
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Background/Objective : Perivertricular leukomalacia, a common brain white matter lesion in preterm neonates, is a major risk factor for cerebral palsy. Epidemiologic studies have demonstrated an association between infection and perivertricular leukomlacia. The purpose of this study was to determine if ascending intrauterine infection could cause brain white matter lesions in the fetal rabbit.
Study Design : Timed-pregnant rabbits underwent hysteroscopy at 20-21 days of gestation(70 %). Animals were randomly allocated in a ratio of 3 : 1 for inoculation with either E. coli(0.2 ml containing 103 or 104 cfm) or sterile saline. Both groups wee treated with ampicillin-sulbactam(100 mg/kg/day ; Unasyn : Pfizer, Seoul, Korea) every S hour until euthanasia that was performed 5 to 6 days after hysteroscopy. Histological examination of the placenta and fetal brains was conducted.
Results : Forty animals were underwent hysteroscopy ; 31 were inoculated with E. coli and 9 with sterile saline. E. coli inoculated animals had a higher rate of intrauterine infection and fewer live fetuses at euthanasia than those in the saline group. Histological evidence of brain white matter damage was found more frequently in fetuses with intrauterine infection than in those without infection[8 %(12/159) vs. 0%(0/103) ;p<0.0005)1. Evidences of white matter damage included increased karvorrhexis, rarefaction and disorganization of white matter. Apoptosis was demonstrated in areas of white matter damage by immunohistochemistry.
Conclusion : Experimental ascending intrauterine infection can cause fetal brain white matter lesions.
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